In real-time quantitative PCR, light emission will result or will become visible in a person who has a hepatitis C infection when:

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In real-time quantitative PCR (qPCR), the probe oligonucleotide is designed to hybridize to a specific target sequence within the DNA or RNA being amplified. During the PCR amplification process, the probe is degraded by the 5' to 3' exonuclease activity of Taq polymerase as it extends the primer and synthesizes the new DNA strand. The degradation of the probe releases a fluorescent dye that was quenched when the probe was intact. This increase in fluorescence can be measured in real-time, and it is directly proportional to the amount of target nucleic acid in the sample, making it visible in cases such as a hepatitis C infection when the viral RNA is being amplified.

In Real-Time Quantitative PCR, Light Emission Will Result or Will