Exhibit 6A This is a reaction going on in your muscle cells right this very minute:
The enzyme triose phosphate isomerase catalyzes this reaction in the forward direction as part of the glycolytic pathway.It follows simple Michaelis-Menten kinetics:
Typical cellular concentrations: triose phosphate isomerase = 0.1 nM
Dihydroxyacetone phosphate = 5 µM glyceraldehyde-3-phosphate = 2 µM
Refer to Exhibit 6A."Hindrate" is an inhibitor of triose phosphate isomerase.When it is added to cells at a concentration of 0.1 nM,the enzyme's K_M for the substrate is unchanged,but the apparent V_max is altered to 50 nM/sec.

Question

Exhibit 6A This is a reaction going on in your muscle cells right this very minute:

The enzyme triose phosphate isomerase catalyzes this reaction in the forward direction as part of the glycolytic pathway.It follows simple Michaelis-Menten kinetics:

Typical cellular concentrations: triose phosphate isomerase = 0.1 nM
Dihydroxyacetone phosphate = 5 µM glyceraldehyde-3-phosphate = 2 µM
Refer to Exhibit 6A."Hindrate" is an inhibitor of triose phosphate isomerase.When it is added to cells at a concentration of 0.1 nM,the enzyme's K_M for the substrate is unchanged,but the apparent V_max is altered to 50 nM/sec.

Quizplus · Accepted Answer

Hindrate is altering the maximum turnover rate of triose phosphate isomerase without changing the affinity of the enzyme for its substrate. This indicates that it is a noncompetitive inhibitor.

Exhibit 6A This Is a Reaction Going on in Your