The major difference between RNA initiation in eukaryotes and prokaryotes is the number of factors involved in the process.

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In eukaryotes, the process of RNA initiation involves a complex set of transcription factors and a multi-subunit RNA polymerase, making it more intricate than in prokaryotes, which typically utilize a simpler system with fewer factors for RNA polymerase to initiate transcription.

The Major Difference Between RNA Initiation in Eukaryotes and Prokaryotes