In the synthesis of DNA in Escherichia coli A) a single-strand nick in the template DNA gives rise to a swivel point just in advance of the replication fork B) Mn²⁺ is required C) all enzymes involved are single polypeptide chains D) neither the template DNA nor the daughter DNA molecules exhibit supercoiling

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During DNA replication in Escherichia coli, topoisomerase creates a single-strand nick in the template DNA, which serves as a swivel point to relieve the tension from supercoiling ahead of the replication fork, allowing the replication machinery to proceed efficiently.

In the Synthesis of DNA in Escherichia Coli