The Kirby-Bauer test uses an agar surface seeded with the test bacterium, and small discs containing a specific concentration of several drugs are placed on the surface.

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The Kirby-Bauer test indeed uses an agar surface seeded with the test bacterium, and small discs containing a specific concentration of several drugs are placed on the surface. The drugs diffuse into the surrounding agar, and a clear zone of inhibition around each disc indicates the antibiotic's effectiveness against the tested organism.

The Kirby-Bauer Test Uses an Agar Surface Seeded with the Test