If you observe rod-shaped, pink cells on a slide that had just been Gram stained, you can assume that their cell envelope contains endotoxin.

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Gram-negative bacteria have a cell envelope that contains endotoxin, which is released when the bacteria dies and the cell wall is disrupted during staining. Pink, rod-shaped cells are typically Gram-negative bacteria, so it is safe to assume their cell envelope contains endotoxin.

If You Observe Rod-Shaped, Pink Cells on a Slide That