You have made a smear of a bacterial culture and have performed the Gram stain on it.Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction, but instead are light purple.Your staining procedure was performed correctly.What is your best explanation as to why the bacteria have stained this way?

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Quizplus · Accepted Answer

The light purple staining suggests that the bacterial cells did not retain the crystal violet dye during the staining process, which indicates that the cells have a thinner peptidoglycan layer in their cell wall. This is often characteristic of gram-negative bacteria, which have an additional outer membrane that can interfere with the uptake of the crystal violet stain.

You Have Made a Smear of a Bacterial Culture and Have