You need to determine if a pure culture of bacteria is gram-positive or gram-negative, but you've just spilled your only solution of crystal violet so you can't do a Gram stain.You decide to try a lysozyme treatment on a sample of each culture and then examine the samples under the microscope.The expected result is

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Lysozyme is an enzyme that breaks down the peptidoglycan layer in the cell wall of bacteria, causing them to lyse. Gram-negative bacteria have a thinner peptidoglycan layer compared to gram-positive bacteria, which makes them more resistant to lysozyme treatment. Therefore, gram-negative bacteria will be less affected by the lysozyme treatment, while gram-positive bacteria will be lysed and appear as debris. Thus, the expected result is that gram-positives will lyse and appear as debris, while gram-negatives will be unaffected.

You Need to Determine If a Pure Culture of Bacteria