To make NPH intermediate-acting insulin,protamine and zinc are added.Protamine was originally taken from the semen of a river trout.

Question

Quizplus · Accepted Answer

Protamine is a type of protein that can neutralize the negative charge of DNA and is commonly used to stabilize insulin formulations. It was originally isolated from fish sperm, including salmon and herring, but can now be produced synthetically or through recombinant DNA technology. Zinc is also added to NPH insulin to prolong its duration of action.

To Make NPH Intermediate-Acting Insulin,protamine and Zinc Are Added