The first 3 to 10 meters of a capillary column serves as a guard column and retention gaps.Guard columns:
A)improve peak shape by separating volatile solvent from less volatile solutes prior to chromatography.
B)divert a portion of the sample to waste to improve peak shape.
C)accumulate nonvolatile substances to prevent column contamination
D)begin separation by retaining less volatile solutes from volatile solutes.
E)increase the number of theoretical plates,reducing plate height.

Question

Quizplus · Accepted Answer

Guard columns accumulate nonvolatile substances to prevent contamination of the main analytical column, thereby extending its life and maintaining performance. They do not directly participate in the separation process or improve peak shape by separating compounds before analysis.

The First 3 to 10 Meters of a Capillary Column