Normal-phase chromatography uses a____________________ stationary phase and a____________________ mobile phase.The more____________________ the mobile phase becomes,the stronger the eluent strength.
A)polar;polar;polar
B)nonpolar;nonpolar;polar
C)polar;nonpolar;nonpolar
D)polar;nonpolar;polar
E)nonpolar;polar;nonpolar

Question

Quizplus · Accepted Answer

Normal-phase chromatography uses a polar stationary phase and a nonpolar mobile phase. The more polar the mobile phase becomes, the stronger the eluent strength. This means that as the mobile phase becomes more polar, it is better able to compete with the stationary phase for binding to the sample molecules, leading to stronger elution. Choice D is the only option that matches these criteria.

Normal-Phase Chromatography Uses A____________________ Stationary Phase and A____________________ Mobile Phase

Normal-Phase Chromatography Uses A Stationary Phase and A Mobile Phase