You are growing a population of cells that appear to be resistant to G418,a chemical that is normally toxic to cells.Upon further analysis of the cells' genome you find that it contains a genomic sequence that encodes the protein neomycin phosphotransferase.What would you do to test if this protein confers resistance to the G418 chemical?
A)determine if the cells are also making β-galactosidase
B)tag the protein with green fluorescent protein to see if it is degraded by G418
C)label a fragment of the gene to see where it is expressed
D)clone the gene into a G418-sensitive cell line,then treat these cells with G418

Question

Quizplus · Accepted Answer

Cloning the gene into a G418-sensitive cell line and treating these cells with G418 will allow us to test if the neomycin phosphotransferase protein is conferring resistance to the chemical. If the cells become resistant to G418 after the introduction of the gene, we can conclude that the protein is responsible for conferring the resistance. The other options do not directly test for the function of the neomycin phosphotransferase protein in conferring resistance to G418.

You Are Growing a Population of Cells That Appear to Be