Modifying the DNA sequence of a gene of interest by appending to it a DNA sequence that encodes a stretch of amino acids recognized by a known monoclonal antibody is called:
A)epitope tagging.
B)in situ hybridization.
C)polymerase chain reaction.
D)next-generation sequencing.

Question

Quizplus · Accepted Answer

The process described in the question is epitope tagging, which involves modifying a gene by appending a DNA sequence that encodes a short stretch of amino acids, known as an epitope, to the protein of interest. This enables detection and purification of the protein using a specific monoclonal antibody that recognizes the epitope sequence. In situ hybridization involves the use of labeled probes to detect RNA molecules in cells or tissues, PCR is a technique used to amplify specific DNA sequences, and next-generation sequencing is a high-throughput method for analyzing DNA or RNA sequences.

Modifying the DNA Sequence of a Gene of Interest by Appending