The purpose of treating tissue from an embryonic chick with trypsin and EDTA when generating a primary cell culture is to:
A) break down proteins present in the serum so the cells can use the amino acids for energy.
B) cleave any cell surface proteins so you can grow the chick cells as a suspension culture for Difficulty: Easy use in an experiment.
C) break the protein–protein interactions that hold cells together in a tissue.
D) prevent cell senescence.

Question

Quizplus · Accepted Answer

Trypsin and EDTA are used to break down the extracellular matrix that holds cells together in a tissue. This allows the cells to be dissociated and cultured as a primary cell culture.

The Purpose of Treating Tissue from an Embryonic Chick with Trypsin