Which of the following allows one to circumvent the theoretical resolution of the microscope?
A)total internal reflection fluorescence microcopy
B)photo-activated localization microscopy
C)indirect immunofluorescence microscopy
D)double-label fluorescence microscopy

Question

Quizplus · Accepted Answer

Photo-activated localization microscopy (PALM) allows one to circumvent the theoretical resolution of the microscope by using photoactivatable fluorescent proteins to light up individual molecules in a sample, which can then be reconstructed into a high-resolution image. This technique has a resolution of approximately 10-20 nm, which is much higher than the theoretical limit of traditional fluorescence microscopy. A, C, and D do not allow circumvention of the theoretical resolution limit.

Which of the Following Allows One to Circumvent the Theoretical