Which of the following techniques would you need to use in order to determine the size of the large and small ribosomal subunits in E.coli?
A)X-ray crystallography
B)plaque assay
C)centrifugation
D)hybridization

Question

Quizplus · Accepted Answer

Centrifugation is a technique that can separate cellular components based on their size and density. By subjecting E.coli lysates to gradient centrifugation, the large and small ribosomal subunits can be separated and their sizes can be determined. X-ray crystallography is a technique that could be used to determine the structure of the ribosomal subunits but not their size. Plaque assay and hybridization are techniques used in microbiology and molecular biology respectively and are not relevant for determining ribosomal subunit size in E.coli.

Which of the Following Techniques Would You Need to Use