When the CAU anticodon of a tRNAᴹᵉᵗ was modified to UAC,the anticodon for tRNAⱽᵃˡ,valine aminoacyl-tRNA synthetase recognized the altered tRNAᴹᵉᵗ and added valine rather than methionine to it.When the converse modification was made,the altered tRNAⱽᵃˡ containing a CAU anticodon (rather than UAC)was recognized and activated by methionine aminoacyl-tRNA synthetase.What do these data suggest about the mechanism by which aminoacyl-tRNA synthetases recognize their cognate tRNAs?
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