An investigator wants to use FRAP to quantify the lateral movement of a specific plasma membrane protein,but to do so this investigator must first:
A)focus a laser on one region of the cell surface.
B)measure the intensity of the fluorescence of the cell surface.
C)label the cell with a fluorescent reagent that binds specifically to the cell surface.
D)add detergents to make the cell surface more fluid.

Question

Quizplus · Accepted Answer

FRAP (Fluorescence Recovery After Photobleaching) involves labeling the cell with a fluorescent reagent that binds specifically to the cell surface. The fluorescently labeled protein is then bleached using a laser, and the rate at which the protein moves back into the bleached area is measured to determine lateral movement. Therefore option C is the correct choice. Options A and B are not applicable to FRAP, and option D is incorrect as adding detergents would disrupt the cell membrane and not accurately measure the lateral movement of the specific plasma membrane protein.

An Investigator Wants to Use FRAP to Quantify the Lateral