When using immunofluorescence microscopy with labeled Annexin V antibodies you found that there is a change in the distribution of the fluorescence when the cells were treated with tumor necrosis factor,an apoptosis-inducing agent.Your data show that this increase in Annexin V fluorescence increases specifically in the:
A)mitochondria.
B)exoplasmic face of the plasma membrane.
C)Golgi.
D)endoplasmic reticulum.

Question

Quizplus · Accepted Answer

Annexin V is a protein that binds to phosphatidylserine, which is normally located on the cytoplasmic face of the plasma membrane. However, during apoptosis, there is a translocation of phosphatidylserine to the outer exoplasmic face of the plasma membrane, which can be detected by Annexin V binding. Therefore, the increase in Annexin V fluorescence specifically at the plasma membrane suggests that apoptosis has been induced.

When Using Immunofluorescence Microscopy with Labeled Annexin V Antibodies You