Which one of the following techniques would be best suited to follow how a thyroxine-bound receptor translocates from the cytoplasm to its DNA response element?
A)in situ hybridization and a radioactive fragment of the DNA response element
B)fluorescence microscopy and a GFP-tagged receptor fusion protein
C)pulse-chase radiolabeling
D)none of the above

Question

Quizplus · Accepted Answer

Fluorescence microscopy and a GFP-tagged receptor fusion protein would be the best choice for following how a thyroxine-bound receptor translocates from the cytoplasm to its DNA response element because it allows real-time visualization of the movement of the receptor in living cells. In situ hybridization and a radioactive fragment of the DNA response element would not provide information on the receptor's movement, while pulse-chase radiolabeling is a technique used to follow the synthesis and degradation of proteins, not their movement within cells.

Which One of the Following Techniques Would Be Best Suited