In an experiment you have used recombinant DNA technology to create hnRNP C protein tagged with green fluorescent protein and hnRNP A1 protein tagged with red fluorescent protein.What would you expect to see when the proteins are expressed and visualized in Xenopus cells?

Question

Quizplus · Accepted Answer

Since hnRNP C is predominantly found in the nucleus, we would expect to see green fluorescence in the nucleus indicating the presence of the tagged hnRNP C protein. Similarly, hnRNP A1 is also known to shuttle between the nucleus and cytoplasm, so we would expect to see both red fluorescence in the nucleus and cytoplasm indicating the presence of the tagged hnRNP A1 protein in both compartments. Therefore, the best choice is D, green and red fluorescence in the nucleus.

In an Experiment You Have Used Recombinant DNA Technology to Create