Formation of a precipitin arc between a well containing a test specimen and a trough containing antiserum indicates:

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Formation of a precipitin arc between a well containing a test specimen and a trough containing antiserum indicates the presence of the protein specific to the antiserum.Lack of a precipitin arc indicates that a detectable amount of the protein is not present in the test specimen.The size,location,and shape of the precipitin arc compared with the control are indications of the amount of protein in the test specimen.When protein concentrations are below normal,precipitin arcs are shortened and are located farther from the antiserum trough than the corresponding arc in the control.When protein concentrations are above normal,precipitant arcs are thicker and are located closer to the antiserum trough than the control.

Formation of a Precipitin Arc Between a Well Containing a Test