A blood specimen for testing for the presence of cryoglobulins should be:

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To test for the presence of cryoglobulins,blood is collected,placed in warm water,and centrifuged at room temperature.The serum is then put into a graduated centrifuge tube and placed in a 4°-C environment for 7 days.If a gel or precipitate is observed,the tube is centrifuged and the precipitate is washed at 4° C,redissolved at 37° C,and evaluated by double diffusion and immunoelectrophoresis for the content of the cryoglobulin.Newer methods use nephelometry with cold treatment for analysis.

A Blood Specimen for Testing for the Presence of Cryoglobulins