The Deinococci stain Gram-positive but have a layered cell wall and an outer membrane that is more like a Gram-negative organism.

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Deinococci are characterized by a layered cell wall that is similar to the Gram-positive bacteria, but they also have an outer membrane that contains lipopolysaccharides (LPS) like Gram-negative organisms. This unique combination of features makes them difficult to classify using traditional Gram staining methods. However, they are considered to be Gram-positive because they retain the stain after decolorization with alcohol or acetone.

The Deinococci Stain Gram-Positive but Have a Layered Cell Wall