Isoelectric focusing separates proteins based on their isoelectric point (pI), which is the pH at which a protein has no net charge and will not move in an electric field. By using a pH gradient, proteins can be separated based on their pI, with acidic proteins migrating towards the anode and basic proteins migrating towards the cathode.

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Isoelectric Focusing

Question 22

Multiple Choice

Isoelectric focusing

A) separates proteins based on the pH at which they are electrically neutral.
B) separates proteins based on their size and shape.
C) directly reveals the identity of unknown proteins in a complex mixture.
D) focuses the data gathered onto a specific protein or ORF.

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