As a newly hired molecular biologist for a company that produces genetically modified seeds,your first project is to ensure that a GM cotton plant carrying a bacterial insecticide gene is expressed in tissues of seedlings as well as mature plants.To better understand regulation of the gene,you decide to use GFP fusion to detect activity of the promoter and also use GFP fusion to determine the location of the protein in plants tissues at various stages of growth.

Question

Quizplus · Accepted Answer

To study gene expression, one can use GFP (green fluorescent protein) fusion to determine whether the gene or promoter of interest is active at the transcriptional level (by visualizing GFP expression in tissues) and/or translational level (by visualizing the location of the GFP fusion protein within cells). In this case, the project is focused on ensuring that the bacterial insecticide gene is expressed in both seedling and mature cotton plants, and understanding the regulation of the gene. Therefore, the best choice would be A, using GFP fusion to detect activity of the promoter (which would be at the transcriptional level) and also using GFP fusion to determine the location of the protein in plant tissues (which would be at the translational level).

As a Newly Hired Molecular Biologist for a Company That