To best isolate mutant bacteria that are threonine auxotrophs from a larger population,threonine-rich media should be inoculated and any colonies that form are presumptive auxotrophic mutants.

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To isolate threonine auxotrophs, you would use a minimal media that lacks threonine. Auxotrophs require the external addition of threonine to grow, so they would not grow on threonine-deficient media, allowing for their isolation based on the absence of growth.

To Best Isolate Mutant Bacteria That Are Threonine Auxotrophs from a Larger