A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.

Question

Quizplus · Accepted Answer

The resolution of a microscope is determined by the numerical aperture of the objective lens and the wavelength of light used. The smaller the numerical aperture and the longer the wavelength, the lower the resolution. In this case, the numerical aperture is 0.65 and the wavelength is 420 nm, which allows for a resolution of 400 nm. Therefore, two objects 400 nm apart can be distinguished using this microscope.

A Light Microscope with an Objective Lens Numerical Aperture of 0.65