Gram staining divides bacterial species into two groups based on differences in cell wall structure.

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Gram staining is a technique used to differentiate bacteria into two groups based on their cell wall structure, allowing for identification of the bacterium. Gram-positive bacteria have a thick peptidoglycan layer in their cell wall, which retains the crystal violet stain during the staining process, while gram-negative bacteria have a thin peptidoglycan layer and an outer membrane that prevents the crystal violet from binding, causing them to appear pink or red when counterstained with safranin.

Gram Staining Divides Bacterial Species into Two Groups Based on Differences