In transmission electron microscopy,spreading a specimen out in a thin film with uranyl acetate,which does not penetrate the specimen,is called

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The technique described in the question is known as negative staining, where the specimen is spread out in a thin film and stained with a substance that does not penetrate it (in this case, uranyl acetate). This creates a contrast between the specimen and the background, allowing for easier visualization under the electron microscope. Freeze-etching, simple staining, and shadow staining are different techniques in electron microscopy.

In Transmission Electron Microscopy,spreading a Specimen Out in a Thin