To clone a recombinant plasmid,you would
A) insert the plasmid into a virus and inject the virus into a human cell.
B) allow bacteria to take up the plasmid and copy it many times.
C) mix the plasmid with restriction enzymes and nucleotides.
D) use ligase to fuse the ends of the plasmids together.

Question

Quizplus · Accepted Answer

Bacteria can easily take up plasmids through a process called transformation, and once inside the cell, the plasmid can be replicated multiple times. This allows for the production of multiple copies of the recombinant plasmid, resulting in the cloning of the plasmid. Option A is incorrect because viruses are not used for cloning recombinant plasmids. Option C is incorrect because mixing a plasmid with restriction enzymes and nucleotides is a step in the process of creating a recombinant plasmid, but it does not directly result in the cloning of the plasmid. Option D is incorrect because ligase is used to fuse together DNA fragments, but it is not the only step in the process of cloning a recombinant plasmid.

To Clone a Recombinant Plasmid,you Would