What technique would you use to best determine when and in what cells of a given tissue a specific gene is expressed?
A)Northern analysis
B)in situ hybridization
C)RT-PCR
D)Southern analysis

Question

Quizplus · Accepted Answer

In situ hybridization is the best technique to determine the spatial and temporal expression pattern of a specific gene in tissue sections or whole embryos. It involves hybridizing a labeled nucleic acid probe (usually a complementary RNA or DNA) to the target mRNA in the tissue or cell of interest. The location and intensity of the hybridization signal can be observed under a microscope, allowing for the identification of the cells expressing the gene. Northern analysis, RT-PCR, and Southern analysis can all detect gene expression, but they do not provide information on the specific cells or tissues where the gene is expressed.

What Technique Would You Use to Best Determine When and in What