In generating a reporter construct to study gene regulation, the reporter gene introduced replaces
A)$\beta$-galactosidase.
B)a random region of DNA.
C)the cis-acting regulatory DNA sequence.
D)X-gal.
E)the coding region of the gene being studied.

Question

Quizplus · Accepted Answer

The purpose of a reporter construct is to study the regulation of a specific gene. Therefore, the ideal choice of a reporter gene would be one that replaces the coding region of the gene being studied, allowing researchers to monitor the expression of the gene by observing the expression of the reporter gene. Options A, B, and D do not provide any useful information about the regulation of the gene. Option C eliminates the regulatory DNA sequence, which is essential for studying gene regulation. Therefore, choice E is the best option.

In Generating a Reporter Construct to Study Gene Regulation, the Reporter