Mutations altering the amount of protein synthesized from a reporter construct occur most often in which of the following sequences of DNA?
A)the reporter gene
B)cis-acting DNA elements
C)X-gal coding sequence
D)RNA polymerase II gene
E)None of the choices is correct.

Question

Quizplus · Accepted Answer

Cis-acting DNA elements are regulatory sequences that determine the level of transcriptional activity of a gene, including the binding sites for transcription factors and the promoter regions. Mutations in these elements can alter the transcriptional activity of the gene, resulting in changes in the amount of protein synthesized from the reporter construct. Mutations in the reporter gene or X-gal coding sequence would directly affect the function of the reporter construct, while mutations in the RNA polymerase II gene would affect the ability of the cell to transcribe all genes, not just the reporter construct.

Mutations Altering the Amount of Protein Synthesized from a Reporter