Enhancers can be identified by
A) cloning a region of DNA with the putative enhancer in a reporter plasmid and demonstrating an increase in gene expression when the construct is introduced into a eukaryotic cell..
B) sequence comparison with promoter sequences that are already known.
C) introducing two seperate pieces of DNA into a eukaryotic cell.One is a fragment of DNA with the putative enhancer and the other is a plasmid that contains a reporter gene.
D) cloning the putative enhancer into a plasmid and measuring the level of reporter gene expression in a prokaryotic cell.
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