The ChIP technique can be used to identify putative DNA binding sites for various transcription factors since it
A)identifies DNA sequences bound to specific proteins.
B)identifies all sites bound by proteins in the nucleus.
C)identifies the size of different protein-DNA complexes.
D)indetifies proteins that can interact with mRNA.

Question

Quizplus · Accepted Answer

The ChIP (chromatin immunoprecipitation) technique involves the use of antibodies to precipitate DNA fragments bound to specific proteins (such as transcription factors) in the nucleus. Therefore, it can be used to identify DNA sequences that are directly bound to transcription factors, providing putative binding sites for these factors. Option B is incorrect because ChIP does not identify all proteins bound in the nucleus, only those specific ones that are targeted for analysis. Option C is incorrect because the size of protein-DNA complexes is not the focus of ChIP analysis. Option D is incorrect because ChIP does not investigate interactions between proteins and mRNA, but instead focuses on the interaction between proteins and DNA.

The ChIP Technique Can Be Used to Identify Putative DNA