Fifteen strains of bacteria that are auxotrophic for maltose do not complement each other.The mutations in these bacteria map to the same DNA region using transformatin of random fragments of genomic DNA.There are only two unique enzymes in the maltose biosynthetic pathway and all of the mutants have a mutation in the reading frame for one or the other .What does this data suggest?

Question

Quizplus · Accepted Answer

The fact that all 15 mutants have mutations in the reading frame of only two unique enzymes in the pathway suggests that these genes are clustered together and could be part of one operon. The fact that these mutants do not complement each other suggests that all of these genes are required for maltose biosynthesis.

Fifteen Strains of Bacteria That Are Auxotrophic for Maltose Do