Which of the following molecular techniques could a scientist use to help locate genes on cloned fragments of DNA with deletion mutants?
A)in situ hybridization and cross over analysis
B)crossover and Southern blot analysis
C)Southern blot , crossover analysis and in situ hybridization
D)in situ hybridization and Southern blot analysis

Question

Quizplus · Accepted Answer

In situ hybridization can help locate and visualize specific genes on DNA fragments, while Southern blot analysis can help detect the presence or absence of specific DNA sequences, including deleted regions of DNA. Together, these two techniques can help locate genes on cloned fragments of DNA with deletion mutants. Therefore, the best choice is D, which includes both in situ hybridization and Southern blot analysis.

Which of the Following Molecular Techniques Could a Scientist Use