What is one difference between using restriction endonucleases and mechanical shearing of DNA?
A)Restriction endonucleases digest at known specific sites while shearing occurs at random sites.
B)Restriction endonucleases digest at random sites while shearing occurs at known specific sites.
C)Restriction enzymes are a mechanical process while shearing occurs using proteins.
D)Restriction enzymes produce larger fragments on average than shearing.

Question

Quizplus · Accepted Answer

Restriction endonucleases are enzymes that recognize and cleave DNA at specific target sequences. Therefore, they digest DNA at known specific sites. On the other hand, mechanical shearing involves physically breaking DNA at random locations, resulting in fragments of various sizes.

What Is One Difference Between Using Restriction Endonucleases and Mechanical