You Expose a Tube of Bacteria to Ultraviolet Radiation to Induce

You expose a tube of bacteria to ultraviolet radiation to induce mutations.You then spread the bacteria on a plate containing minimal media and wait until separate colonies form.Finally,you use replicate plating to transfer a print of this plate to an additional sterile plate containing minimal media plus a known required nutrient for the bacteria.Based on this protocol,colonies growing on the plate lacking a required nutrient would include what type of cells?

A) only wild-type bacteria
B) only bacteria mutant for the supplied nutrient
C) only mutant bacteria but not those mutant for the supplied nutrient
D) wild-type bacteria and bacteria mutant for the supplied nutrient
E) wild-type bacteria and all mutant bacteria except those mutant for the supplied nutrient