Your research project involves the characterization of a signal transduction pathway,and you would like to identify proteins that may interact with one of the pathway components.Which of the following libraries could you screen for this purpose?
A)an expression library
B)a genomic library
C)a phage library
D)a BAC library

Question

Quizplus · Accepted Answer

An expression library contains a collection of cloned genes that are expressed in a host organism, such as bacteria or yeast. This allows identification of proteins that may interact with a specific pathway component through techniques such as affinity purification or yeast two-hybrid screening. Genomic libraries contain all the genes of an organism, whereas phage libraries contain DNA packaged into phage particles, and BAC libraries are used for cloning large DNA fragments. These libraries are not ideal for identifying protein-protein interactions.

Your Research Project Involves the Characterization of a Signal Transduction