A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene.Which of the following techniques would accomplish this goal?
A)Southern blot
B)northern blot
C)western blot
D)eastern blot

Question

Quizplus · Accepted Answer

Northern blotting is used to detect the mRNA expression pattern of a specific gene. This technique involves separating the RNA on a gel, transferring the RNA onto a membrane, and then hybridizing the membrane with a labeled probe that is specific to the gene of interest. The resulting bands on the membrane will indicate the level of expression of the target gene in different tissues or under different conditions.

A Researcher Identified a New Enzyme and Is Interested in Determining