The structures present in the hay infusions used in experiments on spontaneous generation that made them difficult to sterilize are

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Endospores are highly resistant structures that are formed by certain bacteria when conditions are unfavorable for growth. They are resistant to heat, radiation, and chemicals, which makes them difficult to sterilize. Hay infusions used in experiments on spontaneous generation often contained endospores of bacteria that survive sterilization attempts, leading to the mistaken belief in spontaneous generation.

The Structures Present in the Hay Infusions Used in Experiments