During DNA sequencing,a laser beam scans the fragments on the electrophoresis gel to identify the ____________________.
A) gene sequence by the color it fluoresces
B) end nucleotide by the color it fluoresces
C) useful DNA strands that will be analyzed further
D) gene products that have been fixed by the gel
E) gene products that have been sorted by the addition of a probe

Question

Quizplus · Accepted Answer

During DNA sequencing, particularly in methods like Sanger sequencing, each of the four nucleotides (adenine, thymine, cytosine, guanine) is tagged with a different fluorescent dye. As the fragments pass the laser during electrophoresis, the laser excites these dyes, and the color emitted identifies the end nucleotide of each fragment. This allows for the determination of the sequence of nucleotides in the DNA strand being analyzed.

During DNA Sequencing,a Laser Beam Scans the Fragments on the Electrophoresis