To create a mouse model of the human disease Hemophilia A,in which a nonfunctional factor VIII protein results in a blood clotting deficiency,_____.
A) a cloned mutant FVIII gene is inserted into fertilized mouse eggs, which are then transferred to a foster mother for gestation and birth
B) a cloned normal FVIII gene is inserted into fertilized mouse eggs, which are then transferred to a foster mother for gestation and birth
C) mice with similar, naturally occurring, clotting deficiencies are cloned
D) embryonic stem cells are harvested from mouse blastocysts, and grown in the lab to produce blood cells for experimental research
E) adult mice are infected with bacteria that contain a copy of a mutant human FVIII gene

Question

Quizplus · Accepted Answer

To create a mouse model of Hemophilia A, a nonfunctional factor VIII gene needs to be inserted into the mouse genome. Choice A involves inserting a cloned mutant FVIII gene into fertilized mouse eggs, which allows the gene to be integrated into the mouse genome from the very beginning of development. This method has been successfully used in the past to create mouse models of various human genetic diseases. The other choices either involve using preexisting, naturally occurring clotting deficiencies in mice (C), using embryonic stem cells (D), or infecting adult mice with bacteria (E), which may not accurately mimic the genetic basis of Hemophilia A. Choice B is not recommended, as inserting a functional FVIII gene would not result in an accurate model of the disease.

To Create a Mouse Model of the Human Disease Hemophilia