With the 100× lens, the refractive index of light passing through the specimen is maintained by insertion of __________, with a refractive index comparable to that of glass.

Question

Quizplus · Accepted Answer

To maintain the refractive index of light passing through the specimen with a 100× lens, immersion oil with a refractive index comparable to that of glass is used. This helps to reduce the amount of light lost due to refraction, and provides a clearer image. Water and air have different refractive indices compared to glass, and using them as an immersion medium can result in a distorted image. Bacteria and stain are not suitable for maintaining the refractive index of light passing through the specimen.

With the 100× Lens, the Refractive Index of Light Passing