For SDS electrophoresis, proteins are coated with a reagent to give them a uniform __________ charge so that their distance of migration depends on __________.

Question

Quizplus · Accepted Answer

SDS (sodium dodecyl sulfate) gives a negative charge to proteins, making them uniformly negatively charged. The migration distance of protein in SDS-PAGE only depends on molecular weight because the charge density and shape of the proteins are eliminated.

For SDS Electrophoresis, Proteins Are Coated with a Reagent to Give