In an electrophoretic mobility shift assay, the labeled target fragment is mixed in vitro with purified regulatory protein and subjected to electrophoresis through a __________ polyacrylamide gel.

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A nondenaturing polyacrylamide gel is used in an electrophoretic mobility shift assay (EMSA) because it provides the appropriate pore size to allow the DNA-protein complex to migrate more slowly, resulting in a shift in the band position compared to the unbound DNA fragment. Agarose is a larger pore size gel used for DNA separation but is not suitable for EMSA. Denaturing and DNA gels are not appropriate for this assay. Regulatory gel is not a recognized term in this context.

In an Electrophoretic Mobility Shift Assay, the Labeled Target Fragment