You Have Just Isolated Four Homologous Sequences Through PCR Amplification

You have just isolated four homologous sequences through PCR amplification of specific glycolysis protein genomic sequence from four separate bacterial cultures that you proved to be Escherichia coli through morphological and biochemical laboratory analysis. Unknown #3 looks different from the other three. Is this still an Escherichia coli? How can you explain this difference and what can you deduce about the gene product in comparison with the other three samples?
Unknown #1: A A T T T A C G A T A A A T G G A A A A A T T T C G A T
Unknown #2: A A T T T A C G A T A A A T G G A A A A A T T T C G A T
Unknown #3: A A T G G C C G G C G C G C G G A A A A A T T T C G A T
Unknown #4: A A T T T A C G A T A A A T G G A A A A A T T T C G A T