In real-time quantitative PCR, light emission will result or will become visible in a person who has a hepatitis C infection when:

Question

Quizplus · Accepted Answer

In real-time quantitative PCR, light emission occurs when the oligonucleotide probe, which is labeled with a fluorescent dye, is degraded by the exonuclease activity of Taq polymerase during the PCR process. This degradation separates the dye from a quencher, allowing fluorescence to be detected, indicating the presence of the target sequence.

In Real-Time Quantitative PCR, Light Emission Will Result or Will